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lympholyte cell separation density gradient solution  (Cedarlane)


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    Cedarlane lympholyte cell separation density gradient solution
    Lympholyte Cell Separation Density Gradient Solution, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lympholyte+-h+cell+separation+density+gradient+solution/Lympholyte%C2%AE-1%2E1%2C+Cell+separation+media+1%2E100+%C2%B1+0%2E001+g%2Fml+%4025%C2%B0C/pmc10340516-168-17-24
    Average 93 stars, based on 40 article reviews
    lympholyte cell separation density gradient solution - by Bioz Stars, 2026-10
    93/100 stars

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    Isolation:

    Article Title: Identification of Tissue-Resident Natural Killer and T Lymphocytes with Anti-Tumor Properties in Ascites of Ovarian Cancer Patients.
    Article Snippet: At the end, the single-cell suspension was frozen in fetal bovine serum (FBS; Lonza, Basel, Switzerland) supplemented with 10% of dimethylsulfoxide (DMSO; Lonza) in liquid nitrogen for further analysis. .. The peripheral blood mononuclear cells (PBMCs) were isolated from freshly isolated blood of HGSC patients using a Lympholyte® Cell Separation density gradient solution (Cederlane Laboratories: Burlington, ON, Canada) following the manufacturer’s instructions. ..

    Article Title: Intrahepatic CD69 + Vδ1 T cells re-circulate in the blood of patients with metastatic colorectal cancer and limit tumor progression
    Article Snippet: .. Peripheral blood mononuclear cells obtained from the buffy coats of healthy donors (HDs) or from blood of patients with CLM were isolated by Lympholyte Cell Separation density gradient solution (Cederlane Laboratories, Burlington, Canada) according to the manufacturer’s instructions. .. Liver specimens were dissociated by enzymatic digestion with 2 mg/mL of collagenase D (Roche Diagnostic; Indianapolis, Indiana, USA) in gentleMACS Dissociator (Miltenyi; Bergisch Gladbach, Germany) as previously described.

    Article Title: NKp46-expressing human gut-resident intraepithelial V δ 1 T cell subpopulation exhibits high antitumor activity against colorectal cancer
    Article Snippet: .. Human PBMC were isolated from buffy coats of healthy volunteers (HRH) using Lympholyte Cell Separation density gradient solution (Cederlane Laboratories) according to the manufacturer’s instruction. .. Primary HCoEpic (catalog 2950; ScienCell Research Laboratories) were cultured on poly-L-lysine (Sigma-Aldrich) following manufacturer’s instructions in the specific colonic epithelial cell medium (CoEpiCM, catalog 2951) supplemented with colonic epithelial cell growth supplement (CoEpiCGS, catalog 2952) and 1% P/S/A.

    Article Title: NKG2A expression identifies a subset of human Vδ2 T cells exerting the highest antitumor effector functions.
    Article Snippet: .. Human specimens and cell culture Peripheral bloodmononuclear cells (PBMCs) were isolated from buffy coats of HDs or from freshly isolated blood of patients affected byHCC, GBMandNSCLC through Lympholyte Cell Separation density gradient solution (Cederlane Laboratories; Burlington, Canada) according to the manufacturer’s instruction. .. Cell Reports 37, 109871, October 19, 2021 e3 Freshly isolated HCC liver tissue was dissociated by enzymatic digestion in gentleMACSDissociator (Miltenyi; Bergisch Gladbach, Germany) with 2 mg/mL of collagenase D (Sigma Aldrich; St. Louis, Missouri, USA) for 45 minutes at 37 C/5% CO2.

    Article Title: Distinct responses of newly identified monocyte subsets to advanced gastrointestinal cancer and COVID-19
    Article Snippet: .. Samples were collected in EDTA-coated tubes (BD Vacutainer K2E), and peripheral blood mononuclear cells (PBMCs) were isolated by Lympholyte ® cell separation density gradient solution (Cederlane). .. Any residual erythrocytes were removed via ammonium-chloride-potassium (ACK) Lysing Buffer (Lonza) treatment for 60 sec at room temperature (RT).

    Article Title: Identification of Tissue-Resident Natural Killer and T Lymphocytes with Anti-Tumor Properties in Ascites of Ovarian Cancer Patients
    Article Snippet: At the end, the single-cell suspension was frozen in fetal bovine serum (FBS; Lonza, Basel, Switzerland) supplemented with 10% of dimethylsulfoxide (DMSO; Lonza) in liquid nitrogen for further analysis. .. The peripheral blood mononuclear cells (PBMCs) were isolated from freshly isolated blood of HGSC patients using a Lympholyte ® Cell Separation density gradient solution (Cederlane Laboratories: Burlington, ON, Canada) following the manufacturer’s instructions. ..

    Cell Culture:

    Article Title: NKG2A expression identifies a subset of human Vδ2 T cells exerting the highest antitumor effector functions.
    Article Snippet: .. Human specimens and cell culture Peripheral bloodmononuclear cells (PBMCs) were isolated from buffy coats of HDs or from freshly isolated blood of patients affected byHCC, GBMandNSCLC through Lympholyte Cell Separation density gradient solution (Cederlane Laboratories; Burlington, Canada) according to the manufacturer’s instruction. .. Cell Reports 37, 109871, October 19, 2021 e3 Freshly isolated HCC liver tissue was dissociated by enzymatic digestion in gentleMACSDissociator (Miltenyi; Bergisch Gladbach, Germany) with 2 mg/mL of collagenase D (Sigma Aldrich; St. Louis, Missouri, USA) for 45 minutes at 37 C/5% CO2.



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